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Seed DS-01 Synbiotic Testing Results

Mamavation » Blog » Supplements | Mamavation » Seed DS-01 Synbiotic Testing Results
Seed DS-01 Synbiotic sitting on table

February 24, 2026 //  by Leah Segedie

Mamavation independently sent our highest rated probiotic, Seed DS-01 Daily Synbiotic, to an EPA-certified laboratory testing for contaminants such as heavy metals, PFAS “forever chemicals,” and pesticides. After receiving the results, we have good news to report back. You’ve trusted Mamavation to bring you investigations like safest women’s multivitamins, safest children’s multivitamins, & safest probiotics, now join us for the results of our ongoing testing of Seed DS-01.

Disclosure: Mamavation independently paid for laboratory testing to look for potential contamination in this brand. We will now use any affiliate funds we receive from purchases of Seed to pay ourselves back for this testing. This post contains affiliate links. Mamavation receives a small portion of sales. We thank you for your support! 

Researchers at the lab Testing supplements

Table of Contents

  • Mamavation Sent Seed to the Laboratory to Test for Contaminants
    • Lab Results of Seed Daily Synbiotic DS-01
  • Special Offer to Mamavation Community From Seed
  • More About Seed DS-01 & Bacterial Strains Clinically Tested
    • DS-01 Bacterial Strains
    • Digestive Health
      • Clinical Trial Results
    • Immune Health
      • Clinical Trial Results
    • Skin Health
      • Clinical Trial Results
    • Heart Health
      • Clinical Trial Results
    • Gut Barrier
      • Clinical Trial Results
  • Special Offer to Mamavation Community From Seed

Mamavation Sent Seed to the Laboratory to Test for Contaminants

Mamavation has been scrutinizing and testing Seed DS-01 since 2018. This year, we were not able to budget much for a comprehensive probiotic investigation with dozens of products, but we did want to make sure we sent the same probiotic from our last probiotic investigation to the laboratory for a series of tests.

So, Mamavation sent the Seed DS-01 to an EPA-certified laboratory for testing for contaminants. We tested for four types of heavy metals: lead, cadmium, arsenic, & mercury. We also tested for indications of PFAS “forever chemicals,” glyphosate, AMPA, and phthalates. The results were very positive.

Lab Results of Seed Daily Synbiotic DS-01

Here are the total results we got from the lab, along with some context we have provided below.

  • Indications of PFAS “Forever Chemicals”: non-detect organic fluorine
  • 40 PFAS “Forever Chemical” Assays: Non-detect
  • Glyphosate: Non-detect
  • AMPA (Aminomethylphosphonic Acid): Non-detect (breakdown chemical of glyphosate)
  • 300+ Additional Pesticides: Non-detect
  • Phthalates: 242 ppb total phthalate (Context: this is lower than the lowest of women’s multivitamins)
  • Lead: 0.01 mcg/serving (No prop. 65 warning needed)
  • Cadmium: 0.01 mcg/serving (No Prop. 65 warning needed)
  • Arsenic: 0.03 mcg/serving (No Prop. 65 warning needed)
  • Mercury: Non-detect

Seed DS-01 has been one of the cleanest supplements Mamavation has independently tested. According to the laboratory, no glyphosate, AMPA, 300+ additional pesticides, specific PFAS “forever chemicals,” or organic fluorine (indications of PFAS “forever chemicals”) were detected.

One quick note on phthalates: Mamavation has found phthalates in almost every supplement we have tested, including those we have not yet been able to publish yet. (More supplement consumer studies are coming!) Therefore, we know what lower phthalates look like in the supplement industry right now. We found low levels of phthalates in Seed DS-01. However, note that they were lower than the lowest of women’s multivitamins. Therefore, they would be slotted in our “best” category. We do have one caveat. The only place Mamavation has found non-detect phthalate results in supplements is with some “gummies” and “chews” in children’s multivitamins, but we have not found this to be the case with solid supplements. All solid supplements tested have had phthalate detections thus far. (Including supplements in upcoming consumer studies.) Shining a light on this, with the help of consumers, can only make it better.

The four basic heavy metals our lab tested for were lead, cadmium, arsenic, & mercury. All detections were far below Prop. 65 warning levels. Therefore, Seed DS-01 would not require any warnings regarding heavy metals, according to the State of California, which has the strictest lead restrictions in the country.

Special Offer to Mamavation Community From Seed

Seed is celebrating by offering a special discount to the Mamavation community. Get 30% off your initial month of Seed by using this link.  (This is good till March 31st, and after that time will go back to 20%)

Seed DS-01 Synbiotic being held by woman

More About Seed DS-01 & Bacterial Strains Clinically Tested

The DS-01 is a daily probiotic + prebiotic clinically proven to relieve bloating, gas, and irregularity—plus long-term benefits for immune, skin, and heart health. Seed contains 24 bacterial strains, totaling 53.6 billion AFU. They are tested by Seed for quality, pesticides, and allergens.

Only about 16% of supplements are clinically tested. DS-01 has been clinically proven in 4 gold-standard, double-blind placebo controlled clinical trials. In addition, DS-01’s probiotic strains have been validated in 10, dose-matched gold-standard clinical trials.

These specific bacterial strains, when used together, have been proven in clinical trials to be very beneficial to your gut, immune, skin, and heart health.

DS-01 Bacterial Strains

These are the 24 bacterial strains & species contained in DS-01:

Bifidobacterium longum SD-BB536-JP, Bifidobacterium breve SD-BR3-IT, Lactobacillus plantarum SD-LP1-IT, Lactobacillus rhamnosus SD-LR6-IT, Lactobacillus reuteri SD-LRE2-IT, Bifidobacterium longum HRVD90b-US, Bifidobacterium lactis RD-BS5-IT, Lactobacillus casei HRVD300-US, Bifidobacterium breve HRVD521-US, Bifidobacterium lactis HRVD524-US, Lactobacillus rhamnosus HRVD113-US, Bifidobacterium lactis SD-150-BE, Lactobacillus rhamnosus SD-GG-BE, Lactobacillus reuteri SD-RD830-FR, Bifidobacterium adolescentis SD-BA5-IT, Lactobacillus crispatus SD-LCR01-IT, Lactobacillus fermentum SD-LF8-IT, Lactobacillus plantarum SD-LPLDL-UK, Bifidobacterium lactis SD-MB2409-IT, Lactobacillus salivarius SD-LS1-IT, Bifidobacterium lactis SD-CECT8145-SP, Bifidobacterium longum SD-CECT7347-SP, Lactobacillus casei SD-CECT9104-SP.

Digestive Health

DS-01 is formulated to improve the frequency of your bowel movements, as well as increase stool consistency and comfort during evacuation.

Clinical Trial Results

  • In a 30-day study on select DS-01 strains, adults experiencing gastrointestinal troubles saw significant improvement in bowel movement frequency, consistency, and ease of defecation, compared to placebo.
  • DS-01’s strains also demonstrated improvements in burning, discomfort, sense of complete emptying, and abdominal bloating.

Immune Health

DS-01 promotes a strong, resilient immune system. Dedicated strains support the gut-immune axis, which allows these systems to communicate effectively and strengthen overall immune function.

Clinical Trial Results

  • In a 12-week study using select DS-01 strains, participants showed a reduction in microbial translocation across the intestinal epithelial barrier and favorable circulating Th17:Treg and Th1:Th2 ratios. These findings indicate an appropriately responsive immunologic profile.

Skin Health

DS-01 is formulated with dedicated strains that support the gut-skin axis, which plays a key role in regulating inflammatory pathways between the gut and skin.

Clinical Trial Results

  • A 4-week study found DS-01’s prebiotic significantly reduced wrinkle severity and demonstrated an overall shift in the skin microbiome, indicating a beneficial effect on the gut-skin axis.
  • A 12-week study of adults with functionally and aesthetically bothersome skin showed DS-01 strains not only improved skin appearance, but also raised quality of life scores.

Heart Health

DS-01 is formulated to help maintain healthy, in-range cholesterol and blood pressure levels. Select strains promote the production of beneficial metabolites in the gut, which supports healthy liver function and cholesterol balance.

Clinical Trial Results

  • In a 12-week study, DS-01 strains helped probiotic group participants maintain healthy, baseline LDL-C levels, compared to a placebo group that experienced LDL-C increases.
  • Additionally, the probiotic group demonstrated reinforcement of HDL-cholesterol and evidence of healthy triglyceride management within the 6-12 week timeframe.

Gut Barrier

DS-01 is formulated to strengthen and support a resilient gut barrier. Select strains replenish microflora in the gut in order to help rebuild a weakened microbiome.

Clinical Trial Results

  • In a 12-week study using select DS-01 strains, adults experiencing increased gut permeability showed significant reductions in circulating lipopolysaccharide (LPS), a marker of increased epithelial permeability, the cells which comprise the gut barrier.
  • DS-01® significantly reduced urinary lactulose excretion starting at 7 days and after 3 months of ongoing DS-01 use, compared to the placebo group— indicating both immediate and continued support for gut barrier integrity and function.

Seed DS-01 Synbiotic being held in hand in dark

Special Offer to Mamavation Community From Seed

Seed is celebrating by offering a special discount to the Mamavation community. Get 30% off your initial month of Seed by using this link. 

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Reader Interactions

Comments

  1. Judy Resendes

    February 26, 2026 at 9:15 am

    Please test Dr. Livinggood multivitamin and D3K2
    Thanks

    Reply
  2. Carol vanbramer

    February 24, 2026 at 4:21 pm

    Hello. I was wondering if you could test Standard Process Catalyn, which is a multiple vitamin used by many of your subscribers. Thank you.

    Reply

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